EFEK EKSTRAK ETANOL DAUN BINAHONG (Anredera cordifolia (Tenore) Steenis) SEBAGAI ANTIOKSIDAN PADA LENSA KAMBING DI ISOLASI GLUKOSA

Feriyani Feriyani, Hady Maulanza

Sari


Abstrak: Katarak Merupakan Penyakit Yang Berdampak Pada Kebutaan. Obat Penurun Kadar Sorbitol Memiliki Efek Mencegah Transformasi Glukosa Menjadi Sorbitol Yang Didapatkan Hasil Positif Pada Katarak Diabetes. Penelitian Ini Bertujuan Dengan Menilai Gambaran Kekeruhan Lensa Dan Penangkapan Radikal Bebas Dari Ekstrak Daun Binahong (Anredera Cordifolia (Tenore) Steenis) Sebagai Anti Katarak Dengan Percobaan Pada Lensa Kambing. Penelitian Ini Merupakan Penelitian Eksperimental. Penelitian Ini Dilakukan Preparasi Material Dan Ekstrak Tanaman Preparasi Sampel Daun Anredera Cordifolia (Tenore) Steenis Dan Melakukan Inkubasi Lensa Yang Terbagi Kedalam 5 Kelompok (Lensa Normal Dengan Glukosa 5,5 Mm, Lensa Katarak Diabetes Dengan Glukosa 55 Mm, Lensa Katarak Diabetes Dengan Ekstrak Daun Binahong 100 Μg/Ml, Lensa Katarak Diabetes Dengan Ekstrak Daun Binahong 200 Μg/Ml, Lensa Katarak Diabetes Dengan Vitamin E. Analisis Sampel Dengan Menilai Kekeruhan Lensa, Pengujian Antioksidan Menilai DPPH Dan IC 50. Hasil Penelitian Setiap Lensa Kelompok Eksperimen Menunjukan Perubahan Transparansi Lensa Apabila Dibandingkan Dengan Lensa Normal Serta Nilai IC50 Pada Kelompok Lensa Katarak Diabetes Dengan Ekstrak Etanol Daun Binahong 200 Μg/M Cukup Tinggi Dibandingkan Dengan Kelompok Katarak Diabetes Dengan Vitamin E. Ekstrak Daun Binahong (Anredera Cordifolia (Tenore) Steenis) Menunjukkan Sifat Antioksidan Kuat.


Kata Kunci


Katarak; Lensa; Antioksidan; Radikal Bebas; DPPH

Teks Lengkap:

PDF

Referensi


Fang, H., Hu, X., Wang, M., Wan, W., Yang, Q., Sun, X., Gu, Q., Gao, X., Wang, Z., & Gu, L. (2015). Anti-osmotic and antioxidant activities of gigantol from Dendrobium aurantiacum var. denneanum against cataractogenesis in galactosemic rats. Journal of Ethnopharmacology, 172, 238– 246.

Feriyani, D., Lubis, R. R., Balqis, U., & Maulanza, H. (2021). Anti-Cataract and Plant ExtractsBased Natural Products: A Review.

Feriyani, F., Darmawi, D., Balqis, U., & Lubis, R. R. (2020). The Analysis of Binahong Leaves Potential (Anredera cordifolia) as an Alternative Treatment of Anticataractogenesis. Open Access Macedonian Journal of Medical Sciences, 8(B), 820–824.

Gacche, R. N., & Dhole, N. A. (2011). Profile of aldose reductase inhibition, anti-cataract and free radical scavenging activity of selected medicinal plants: an attempt to standardize the botanicals for amelioration of diabetes complications. Food and Chemical Toxicology, 49(8), 1806–1813.

Gülçin, İ. (2006). Antioxidant and antiradical activities of L-carnitine. Life Sciences, 78(8), 803–811.

Kim, J. S. (2014). Seeds of Cornus officinalis and diabetic cataracts. In Handbook of Nutrition, Diet and the Eye (pp. 451–458). Elsevier.

Kumar, M., Singh, T., Ali, J., & Tyagi, L. K. (2011). In vitro anticataract activity of Zingiber officinale on goat lenses. Int J Pharm Biol Arch, 2(5), 1430–1433.

Kumar, P. P., Ramesh, A., Prasad, K., & Gnananath, K. (2017). Evaluation of anti cataract activity of isolated fractions of albizia procera stem bark chloroform extract: targeting Na+ K+ atpase in goat lenses. Advances in Pharmacology and Toxicology, 18(1), 1.

Langade, D. G., Rao, G., Girme, R. C., Patki, P. S., & Bulakh, P. M. (2006). In vitro prevention by ACE inhibitors of cataract induced by glucose. Indian Journal of Pharmacology, 38(2), 107.

Mienie, L. J., Bergh, J. J., & Van der Schyf, C. J. (2004). Acetyl-L-carnitine prevents total body hydroxyl free radical and uric acid production induced by 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) in the rat. Life Sciences, 75(10), 1243–1253.

Miksusanti, M., & Elfita, E. (2012). Aktivitas antioksidan dan sifat kestabilan warna campuran ekstrak etil asetat kulit buah manggis (Garcinia mangostana L.) dan kayu secang (Caesalpinia sappan L.). Jurnal Penelitian Sains (JPS), 15(2), 15213– 15260.

Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Technol, 26(2), 211– 219.

Onkaramurthy, M., Veerapur, V. P., Thippeswamy, B. S., Reddy, T. N. M., Rayappa, H., & Badami, S. (2013). Antidiabetic and anti-cataract effects of Chromolaena odorata Linn., in streptozotocin-induced diabetic rats. Journal of Ethnopharmacology, 145(1), 363–372.

Oyebode, O., Kandala, N.-B., Chilton, P. J., & Lilford, R. J. (2016). Use of traditional medicine in middle-income countries: a WHO-SAGE study. Health Policy and Planning, 31(8), 984–991.

Pintor, J. (2012). Sugars, the crystalline lens and the development of cataracts. Biochem Pharmacol, 1(4), 1–3.

Ribeiro, A. P., Silva, M. L., Rosa, J. P., Souza, S. F., Teixeira, I. A. M. A., & Laus, J. L. (2009). Ultrasonographic and echobiometric findings in the eyes of Saanen goats of different ages. Veterinary Ophthalmology, 12(5), 313–317

Somani, G., & Sathaye, S. (2015). Bioactive fraction of Saraca indica prevents diabetes induced cataractogenesis: An aldose reductase inhibitory activity. Pharmacognosy Magazine, 11(41), 102. 18.

Sychev, Y. V, Zepeda, E. M., & Lam, D. L. (2017). Bilateral cataract formation via acute spontaneous fracture of the lens following treatment of hyperglycemic hyperosmolar syndrome. American Journal of Ophthalmology Case Reports, 7, 66–69. 19.

Thiraphatthanavong, P., Wattanathorn, J., Muchimapura, S., Thukham-Mee, W., Wannanon, P., Tong-Un, T., Suriharn, B., & Lertrat, K. (2014). Preventive effect of Zea mays L.(purple waxy corn) on experimental diabetic cataract. BioMed Research International, 2014. 20.

Zhang, J. H., Ramke, J., Mwangi, N., Furtado, J., Yasmin, S., Bascaran, C., Ogundo, C., Jan, C., Gordon, I., & Congdon, N. (2020). Global eye health and the sustainable development goals: protocol for a scoping review. BMJ Open, 10(3), e035789




DOI: https://doi.org/10.33024/jikk.v10i3.9541

Refbacks

  • Saat ini tidak ada refbacks.


##submission.copyrightStatement##

##submission.license.cc.by-nc4.footer##

Pendidikan Dokter Universitas Malahayati Lampung


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.