Perbandingan Aktivitas Antibakteri Ekstrak Daun Dan Buah Jambu Biji (Psidium guajava L.) Terhadap Enteropathogenic Escherichia Coli (EPEC) Penyebab Diare

Syerli Saputri, Debi Arivo, Tusy Triwahyuni, Tessa Sjahriani

Abstract


Diare yang disebabkan oleh Enteropathogenic Escherichia coli (EPEC) masih menjadi tantangan kesehatan masyarakat, terutama di negara berkembang. Resistensi antibiotik mendorong pencarian alternatif antibakteri berbasis tanaman obat. Jambu biji (Psidium guajava L.) dikenal secara tradisional untuk mengatasi diare, tetapi perbandingan aktivitas ekstrak daun dan buahnya terhadap EPEC masih terbatas. Penelitian ini bertujuan membandingkan pola sensitivitas EPEC terhadap ekstrak etanol daun dan buah jambu biji pada konsentrasi 25%, 50%, 75%, dan 100% menggunakan metode difusi cakram Kirby-Bauer. Ekstrak daun menghasilkan zona hambat 3,83 mm, 6,33 mm, 9,17 mm, dan 13,17 mm, sedangkan ekstrak buah menghasilkan 2,33 mm, 3,33 mm, 3,83 mm, dan 5,50 mm. Analisis One-way ANOVA menunjukkan perbedaan signifikan antar kelompok (F(1,22)=14,748; p<0,001). Ekstrak daun menunjukkan aktivitas antibakteri lebih tinggi dan berpotensi sebagai antibakteri alami terhadap EPEC. Temuan ini diduga berkaitan dengan kandungan metabolit sekunder daun, seperti tanin, flavonoid, dan minyak atsiri, yang berperan menghambat pertumbuhan bakteri secara lebih efektif.


Keywords


Aktivitas antibakteri; Patogen Diare; Ekstrak Daun Jambu Biji; Tanaman obat; Antimikroba alami

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DOI: https://doi.org/10.33024/jmm.v10i2.25287

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