EFEKTIVITAS TABLET KOMBINASI EKSTRAK HERBA PEGAGAN (Centella asiatica L.) DAN EKSTRAK BROKOLI (Brassica oleracea L. Var. Italica) SEBAGAI ANTIDEMENSIA PADA MENCIT JANTAN (Mus musculus)
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Background : Centella asiatica contains active compounds such as asiaticoside, asiatic acid, and flavonoids, while broccoli contains sulforaphane, glucosinolates, and flavonoids. These compounds exhibit antidementia activity by enhancing cognitive function, improving memory, and protecting neurons from oxidative damage.
Purpose : This study aimed to determine the effectiveness and optimal dosage of a combination tablet containing Centella asiatica herb extract and broccoli extract as an antidementia agent in male albino mice.
Methods : The research employed a preclinical experimental design using animal models and the Y-maze method. A total of 25 male mice were divided into five groups: dose I (2,73 mg/20 g body weight), dose II (5,46 mg/20 g body weight), piracetam (11,2 mg/20 g body weight) as the positive control, hyoscine butylbromide (0,03 mg/20 g body weight) as the negative control, and Na-CMC 0.35 mL/20 g body weight as the normal control. All treatments were administered orally for seven consecutive days. On day seven, the mice were subjected to the Y-maze test, and the percentage of spontaneous alternation.
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Carrion, C., Folkvord, F., Anastasiadou, D., & Aymerich, M. (2018). Cognitive Therapy for Dementia Patients: A Systematic Review. Dementia and Geriatry Cognitive Disorders, 46(1–2), 1–26.
Hein, Z. M., Gopalakrishna, P. K., Kanuri, A. K., Thomas, W., Hussan, F., Naik, V. R., & Vishnumukkala, T. (2025). Centella asiatica: advances in extraction technologies, phytochemistry, and therapeutic applications. Life. 15(7), 1081.
Khalisatunnada., Sunaryo, H., & Prastiwi, R. (2023). Uji Aktivitas Ekstrak Etanol 96% Buah Stroberi Terhadap Peningkatan Memori Spasial Pada Tikus Model Demensia. Jurnal Locus: Penelitian & Pengabdian, 2(8), 796–809.
Kraeuter, A. K., Guest, P. C., & Sarnyai, Z. (2018). The Y-maze for assessment of spatial working and reference memory in mice. In Pre-clinical models: Techniques and protocols (pp. 105-111). Springer New York.
Kusuma, M. A., Sakinah, E. N., & Dewi, R. (2017). Efek Hepatoprotektif Ekstrak Etanol Brokoli (Brassica oleracea L. var. italica) terhadap Kerusakan Histologis Sel Hati Tikus Wistar yang Diinduksi DMBA (Hepatoprotective Effect of Ethanolic Extract of Broccoli (Brassica oleracea L. var. italica) on the Hi. Pustaka Kesehatan, 5(1), 6–11.
Nagraj, G. S., Chouksey, A., Jaiswal, S., & Jaiswal, A. K. (2020). Broccoli. In Nutritional composition and antioxidant properties of fruits and vegetables. Academic Press, 5–17.
Navarro-Hortal, M. D., Romero-Márquez, J. M., López-Bascón, M. A., Sánchez-González, C., Xiao, J., Sumalla-Cano, S., & Quiles, J. L. (2024). In vitro and in vivo insights into a broccoli byproduct as a healthy ingredient for the management of Alzheimer’s disease and aging through redox biology. Journal of Agricultural and Food Chemistry, 72(10), 5197-5211.
Nihaya, A., Rahminiwati, M., & Rustiani, E. (2016). Kajian Efek Anti Demensia Ekstrak Brokoli (Brassica oleracea L. Var. Italica Plenck) Dan Pegagan (Centella asiatica L. Urban) Pada Mencit yang Diinduksi Skopolamin. Jurnal Farmasi, 1-5., 1–5.
Reubun, Y. T. A., Kumala, S. S., Setyahadi., & Simanjuntak. (2020). Penghambatan Enzim Asetilkolinesterase dari Ekstrak Herbal Pegagan (Centela asiatica (L.) Urb), Ekstrak Daun Kelor (Moringa oleifera Lam.) dan Kombinasinya. Jurnal Farmasi Indonesia, 17(02), 451–458.
Sehar, U., Rawat, P., Reddy, A. P., Kopel, J., & Reddy, P. H. (2022). Amyloid beta in aging and Alzheimer’s disease. International Journal of Molecular Sciences, 23(21), 12924.
Suryanti, I. (2021). Pengaruh Ekstrak Pegagan (Centella asiatica) Terhadap Daya Ingat Mencit (Mus musculus) yang Diinduksi Streptozotocin (STZ). Doctoral Dissertation, Universitas Islam Negeri Maulana Malik Ahmad.
Sutardi. (2016). Kandungan Bahan Aktif Tanaman Pegagan dan Khasiatnya untuk Meningkatkan Sistem Imun Tubuh. Jurnal Penelitian Dan Pengembangan Pertanian, 35(3), 121-130.
Wang, S., Liu, H. Y., Cheng, Y. C., & Su, C. H. (2021). Exercise dosage in reducing the risk of dementia development: Mode, duration, and intensity—A narrative review. International Journal of Environmental Research and Public Health, 18(24).
Wardani, N. D., Hadiati, T., & Rachmawati, R. (2021). BPSD (Behavioral & Psychological Gejala Demensia) dan Penatalaksanaannya. Semarang. Fakultas Kedokteran Universitas Diponegoro.
Zhang, T., Kim, M. J., & Wu, X. (2022). Long-Term Effect of Porcine Brain Enzyme Hydrolysate Intake on Scopolamine-Induced Memory Impairment in Rats. Int. J. Mol. Sci, 23, 3361.
DOI: https://doi.org/10.33024/jfm.v9i2.24725
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