Analysis Of The Number Of Bacteria In Baby Bottles And The Incidence Of Diarrhea In Toddlers

Nurhasanah Nurhasanah* -  Universitas Muhammadiyah Ahmad Dahlan Cirebon, Indonesia
Nunung Nurjanah -  Universitas Muhammadiyah Ahmad Dahlan Cirebon, Indonesia
Eka Ratnasari -  Universitas Muhammadiyah Ahmad Dahlan Cirebon, Indonesia
Azlillah Azlillah -  Universitas Muhammadiyah Ahmad Dahlan Cirebon, Indonesia
DOI : 10.33024/jkm.v11i10.23177

Latar Belakang Diare merupakan salah satu penyebab morbiditas dan mortalitas pada balita di seluruh dunia. Data World Health Organization (2023) kejadian diare mencapai 10% dari seluruh kematian anak dibawah lima tahun. Kasus diare Indonesia 2022 prevalensi mencapai 10,2% (Kemenkes RI, 2023). Menurut Survey Kesehatan Indonesia (2023) prevalensi diare pada bayi ≤ 1 tahun 6,4% dan pada Balita 1-4 tahun mencapai 7,4% pada kelompok usia 12-59 bulan terdapat 5,8% kasus. Kota Cirebon mendapatkan 8.563 kasus diare dan 30.706 kasus diare di Kabupaten Cirebon (2021). Tingginya angka ini menjadikan diare sebagai salah satu penyebab kematian balita setelah pneumonia. Salah satu penyebab utama penularan agen penyebab diare adalah melalui kontaminasi botol susu yang dapat menyebabkan infeksi gastrointestinal.

Tujuan Penelitian menganalisis hubungan bakteri pada botol susu dengan kejadian diare pada balita. Metode penelitian ini merupakan Penelitian ini merupakan penelitian kuantitatif observassional analitik dengan pendekatan cross sectional. Tahapan penelitian ini dilakukan mulai dari uji laboratorium swab botol susu, dan pengumpulan data kejadian diare pada balita dalam 2 bulan terakhir. Populasi adalah seluruh Ibu Balita (8-24 bulan) Sampel menggunakan Acidental Sampling dengan pengambilan sampel dibatasi pada sampel minimal korelasi sebanyak 30 ibu balita. Teknik analisis data yang digunakan adalah analisis univariat dan bivariate dengan bantuan SPSS.

Hasil penelitian menunjukkan jumlah bakteri dengan kejadian diare diperoleh p-value = 0,200 (≥ 0,05). Kesimpulan tidak terdapat hubungan antara jumlah bakteri botol susu dengan kejadian diare. Saran menjaga kebersihan botol susu dengan mencuci dan mensterilkan secara benar serta tetap memberikan ASI eksklusif untuk meningkatkan daya tahan tubuh anak.

 

Kata kunci: Bakteri, Balita, Bayi,  Botol Susu, Diare

 

ABSTRACT

 

Background Diarrhea is one of the causes of morbidity and mortality in toddlers worldwide. Data from the World Health Organization (2023) shows that diarrhea accounts for 10% of all deaths in children under five years of age. The prevalence of diarrhea cases in Indonesia in 2022 reached 10.2% (Ministry of Health of the Republic of Indonesia, 2023). According to the Indonesian Health Survey (2023), the prevalence of diarrhea in infants ≤ 1 year was 6.4% and in toddlers 1-4 years old reached 7.4%, with 5.8% of cases in the 12-59 month age group. Cirebon City experienced 8,563 cases of diarrhea and Cirebon Regency 30,706 cases (2021). These high numbers make diarrhea one of the leading causes of infant mortality after pneumonia. One of the main causes of transmission of diarrhea-causing agents is through contaminated milk bottles, which can cause gastrointestinal infections.

The purpose of this study was to analyze the relationship between bacteria in baby bottles and diarrhea in toddlers. This study used a quantitative, observational, and analytical cross-sectional approach. The research phase began with laboratory testing of baby bottle swabs and data collection on diarrhea in toddlers over the past two months. The population was all mothers of toddlers (8-24 months). The sample used acid-identical sampling, with a minimum sample size of 30 mothers. Univariate and bivariate analyses were used using SPSS.

The results showed that the number of bacteria in baby bottles correlated with diarrhea, with a p-value of 0.200 (≥ 0.05). Conclusion: There is no relationship between the number of bacteria in baby bottles and diarrhea. Suggestions include maintaining bottle hygiene by washing and sterilizing them properly, and continuing to provide exclusive breastfeeding to boost children's immune systems.

 

Keywords: Bacteria, Toddler, Baby, Milk Bottle, Diarrhea

Keywords : Bacteria, Baby Bottle, Infant, Toddler, Diarrhea

  1. Aisyah, F. N. S., Ghiffari, A., Utama, B., & Chairani, L. (2023). Relationship between formula feeding and diarrhea in infants aged 0–6 months. Jurnal Cakrawala Ilmiah. https://doi.org/10.53625/jcijurnalcakrawalailmiah.v2i6.4968
  2. Amalia, F., Nugroho, D., & Rini, S. (2022). "The Number of Bacteria on Eating Utensils and Its Relationship with Diarrheal Incidence in Sleman Regency." Journal of Environmental Health, 11(1), 58–66.
  3. Darmawan, A., Kusdiyah, E., Mulyadi, D., Herlambang, H., Dewi Aurora, W. I., Harahap, A. H., & Moniga, A. F. (2020). Kontribusi higienitas botol susu dan sumber air terhadap kejadian diare pada balita 6–24 bulan di Puskesmas Kenali Besar Kota Jambi. Jambi Medical Journal: Jurnal Kedokteran dan Kesehatan. https://online-journal.unja.ac.id/kedokteran/article/view/19241
  4. Elegbe, I. A., Ojofeitimi, E. O., & Akinola, M. O. (1994). Pathogenic bacteria isolated from infant feeding teats: Contamination of teats used by illiterate and educated nursing mothers in Ile-Ife, Nigeria. JAMA Pediatrics. https://jamanetwork.com/journals/jamapediatrics/fullarticle/510564
  5. Fitriani, N., & Handayani, S. (2021). "The Relationship Between Family Income and Sanitation Quality and Diarrheal Incidence in Toddlers." Cendekia Utama Journal of Nursing and Public Health, 10(1), 42–51.
  6. Juhl, S., Krogfelt, K. A., Kot, W., Nielsen, D. S., & Krych, L. (2023). Used nasogastric feeding tubes from neonates contain infant-specific bacterial profiles. Microorganisms, 11(6), 1365. https://doi.org/10.3390/microorganisms11061365
  7. Kejriwal, M., & Agarwal, R. (2018). Bacteriology of feeding bottles. IP International Journal of Medical Paediatrics and Oncology, 4(1), 9–10. https://doi.org/10.18231/2455-6793.2018.0004
  8. Kondapalli, C. S., & Siddhartha, G. (2017). Anti-microbial susceptibility pattern of infant feeding bottles from bottle-fed infants. International Journal of Contemporary Pediatrics. https://doi.org/10.18203/2349-3291.ijcp20175056
  9. Hidayah, N., & Mulyani, T. (2021). "Maternal Hygiene Behavior and the Incidence of Diarrhea in Toddlers." Journal of Midwifery and Public Health, 9(3), 221–230.
  10. Lanida, B. P., & Farapti, F. (2018). The prevention strategy against the incidence of diarrhea in toddlers through hygiene of milk bottles-feeding. Jurnal Berkala Epidemiologi, 6(3), 244–251. https://doi.org/10.20473/jbe.V6I32018.244-251
  11. Marege, A., Regassa, B., Seid, M., Tadesse, D., Siraj, M., & Manilal, A. (2023). Bacteriological quality and safety of bottle food and associated factors among bottle-fed babies attending pediatric outpatient clinics of Government Health Institutions in Arba Minch, southern Ethiopia. Journal of Health, Population and Nutrition, 42, 46. https://doi.org/10.1186/s41043-023-00387-1
  12. Ministry of Health of the Republic of Indonesia. (2022). Indonesian Health Profile 2022. Jakarta: Ministry of Health of the Republic of Indonesia.
  13. Mohamed, H., Tarhuni, A., Abbeid, H., Abdulati, A., Adel, A., Saleh, F., & Abdullah, W. (2023). Microbial contamination of infant feeding bottles and caregiver compliance to disinfection and sterilization techniques, Pediatric Hospital, Libya. International Journal of Pathogen Research, 12(6), 110–119. https://doi.org/10.9734/ijpr/2023/v12i6259
  14. Lestari, D., Handayani, R., & Putri, A. (2021). "The Relationship between Eating Utensil Hygiene and the Incidence of Diarrhea in Toddlers." Indonesian Journal of Public Health, 16(2), 87–95.
  15. Ningsih, R., & Rahmawati, S. (2020). "Bacterial Contamination in Baby Bottles and Its Relationship with Diarrhea in Toddlers." Indonesian Journal of Environmental Health, 19(1), 45–52.
  16. Paramitha, G. W., Soprima, M., & Haryanto, R. B. (2008). The conduct of mothers as users of milk bottles and the incidence of diarrhea in infants. Makara Journal of Health Research.
  17. Priyantika, D., Martini, M., & Saraswati, L. D. (2017). Gambaran kontaminasi Salmonella spp. pada botol susu dan kejadian diare bayi dan balita. Jurnal Kesehatan Masyarakat, 5(4). https://doi.org/10.14710/jkm.v5i4.18369
  18. Putri, L., & Anisa, R. (2023). "The Relationship Between Baby Bottle Cleanliness and Diarrhea in Toddlers in the Cilimus Community Health Center Work Area, Kuningan Regency." Journal of Nursing and Public Health, 12(2), 134–142.
  19. Sani, S. L., & Achadi, E. L. (2023). Child feeding practice, sanitation hygiene, and other factors of diarrhea in children aged 6–23 months in Indonesia: Analysis of IDHS 2017. Jurnal Biometrika dan Kependudukan, 12(1), 74–83. https://doi.org/10.20473/jbk.v12i1.2023.74-83
  20. Sari, R., Handayani, T., & Rahmah, F. (2022). "The Relationship between Bottle Cleanliness and the Incidence of Diarrhea in Toddlers in the Panjunan Community Health Center, Cirebon City." Jurnal Ilmu Kesehatan Prima, 13(3), 215–224.
  21. Silva, R. S., et al. (2000). Bacterial contamination of the lacteal contents of feeding bottles in metropolitan São Paulo, Brazil. [Journal name]. https://pubmed.ncbi.nlm.nih.gov/9648358/
  22. Susilowati, E., Astuti, Y., & Mulyasih, R. (2023). Scoping review: Diarrhea in toddlers and causing factors. Jurnal Program Mahasiswa Kreatif, 7(1), 130–138. https://pkm.uika-bogor.ac.id/index.php/pkm-p/article/view/1708
  23. Tilves, C., Zhao, H. J., Differding, M. K., Zhang, M., Liu, T., Hoyo, C., Østbye, T., Benjamin-Neelon, S. E., & Mueller, N. T. (2023). Associations of plastic bottle exposure with infant growth, fecal microbiota, and short-chain fatty acids. Microorganisms, 11(12), 2924. https://doi.org/10.3390/microorganisms11122924
  24. Vitiana, D. N. L., Martini, M., & Hestiningsih, R. (2017). Gambaran kejadian diare balita 6-24 bulan dan kontaminasi bakteri Escherichia coli pada penyajian MP ASI lokal di Kelurahan Tandang, Semarang. Jurnal Kesehatan Masyarakat, 5(4). https://doi.org/10.14710/jkm.v5i4.18368
  25. World Health Organization (WHO). (2017). Guidelines on Sanitation and Health. Geneva: WHO.
  26. World Health Organization (WHO). (2020). Infant and Young Child Feeding: Model Chapter for Textbooks for Medical Students and Allied Health Professionals. Geneva: WHO.

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