Efektivitas Ekstrak Air Daun Eucalyptus pellita Terhadap Bakteri Staphylococcus aureus Dan Acinetobacter baumannii Pada Luka

Yesi Indriyani, Dwi Marlina Syukri, Tessa Sjahriani, Mala Kurniati

Sari


Infeksi bakteri seperti Staphylococcus aureus dan Acinetobacter baumannii merupakan komplikasi umum yang menghambat proses penyembuhan luka, terutama pada kondisi kronis dan luka terinfeksi. Penelitian ini bertujuan untuk mengevaluasi efektivitas ekstrak air daun Eucalyptus pellita terhadap pertumbuhan kedua bakteri tersebut. Penelitian dilakukan secara eksperimental laboratorik menggunakan metode difusi agar, dilaksanakan di Hong Sheng Biotech Co., Ltd pada Mei 2025. Ekstrak diperoleh melalui metode maserasi menggunakan pelarut air dan dilanjutkan dengan proses freeze drying. Hasil uji antibakteri menunjukkan bahwa ekstrak menghasilkan zona hambat sebesar 15,6 ± 1,76 mm terhadap S. aureus dan 15,9 ± 2,81 mm terhadap A. baumannii, yang dikategorikan sebagai daya hambat kuat. Selain itu, uji sitotoksisitas menggunakan metode MTT pada sel fibroblas 3T3 menunjukkan efek toksik yang bersifat dosis-dependent, dengan penurunan viabilitas sel signifikan pada konsentrasi ≥ 0,125 mg/mL. Temuan ini menunjukkan bahwa ekstrak air daun Eucalyptus pellita memiliki potensi sebagai agen antibakteri terhadap bakteri penyebab infeksi luka, meskipun efek toksisitas terhadap sel normal perlu menjadi perhatian dalam pengembangan lanjut.


Kata Kunci


Acinetobacter baumannii; Antibakteri; Eucalyptus pellita; antibakteri; Luka; Staphylococcus aureus

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Referensi


Astiani, D. P., Jayuska, A., & Arreneuz, S. (2014). Uji aktivitas antibakteri minyak atsiri Eucalyptus pellita terhadap bakteri Escherichia coli dan Staphylococcus aureus. Jurnal Kehutanan Tropika, 3(3).

Bachir, R. (2016). Antibacterial activity of leaf essential oils of Eucalyptus globulus and Eucalyptus camaldulensis. African Journal of Plant Science, 3(1), 13–18.

Bagus, A., Kuspradini, H., & Rosamah, E. (2021). Evaluation of antibacterial activity and physico-chemical profiles of Eucalyptus pellita essential oil from East Kalimantan. IOP Conference Series: Earth and Environmental Science, 819, 012007.

Bhowmik, P., Modi, B., Roy, P., & Chowdhury, A. (2023). Strategies to combat Gram-negative bacterial resistance to conventional antibacterial drugs: A review. Osong Public Health and Research Perspectives, 14(5), 333–346.

Christina, L., & Richard, S. (2016). Wound management for the 21st century: Combining effectiveness and efficiency. International Wound Journal, 13(2), 5–15.

Cushnie, T. P. T., & Lamb, A. J. (2015). Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents, 26, 343–356.

Dev, S. K., Choudhury, P. K., & Mandal, V. (2019). Antimicrobial, anti-inflammatory and wound healing activity of polyherbal formulation. Biomedicine & Pharmacotherapy, 111, 555–567.

Didem Karakaş, D., Ari, F., & Ünaldi, E. (2017). The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts. Turkish Journal of Biology, 41(5), 919–925. https://doi.org/10.3906/biy-1703-104

Goh, O. Q., Ong, M. T., Leong, J. J. H., & Tan, J. S. (2020). Incidence of chronic wounds in Singapore, a multiethnic Asian country, between 2000 and 2017: A retrospective cohort study using a nationwide claims database. BMJ Open, 10(9), e038405.

Kementerian Kesehatan RI. (2023). Profil Kesehatan Indonesia 2023. Jakarta: Kementerian Kesehatan Republik Indonesia.

Kharisma, G., Wulandari, A., & Pratiwi, D. (2024). Bioactive compounds from Eucalyptus pellita leaf extract using water solvent. Jurnal Analis Farmasi, 9(1), 40–48.

Khazraei, H., Azra, S., & Mozhdeh, S. (2021). In vitro cytotoxicity and apoptotic assay of Eucalyptus globulus essential oil in colon and liver cancer cell lines. Journal of Gastrointestinal Cancer. https://doi.org/10.1007/s12029-021-00601-5

Li, J., & Monje-Galvan, V. (2023). In vitro and in silico studies of antimicrobial saponins: A review. Journal of Molecular Modeling,

(7), 201.

Martinengo, L., Olsson, M., Bajpai, R., Soljak, M., & Majeed, A. (2019). Prevalence of chronic wounds in the general population: Systematic review and meta-analysis of observational studies. Annals of Epidemiology, 29, 8–15.

Mattson, M. P. (2008). Hormesis defined. Ageing Research Reviews, 7(1), 1–7.

Mendanha, S. A., Moura, D. J., Saffi, J., Calloni, C., Schapoval, E. E. S., & Bassani, V. L. (2013). Toxicity of terpenes on fibroblast cells compared to their hemolytic potential and increase in erythrocyte membrane fluidity. Toxicology in Vitro, 27, 323–329. https://doi.org/10.1016/j.tiv.2012.08.022

Mishra, S.K., Rijal, B.P. and Pokhrel, B.M. (2013) ‘Emerging threat of multidrug resistant bugs Acinetobacter calcoaceticus baumannii complex and Methicillin resistant Staphylococcus aureus’. BMC Research Notes. 2–7.

Moges, G. W., Manahelohe, G. M., & Asegie, M. A. (2024). Phenolic, flavonoid contents, antioxidant, and antibacterial activity of selected Eucalyptus species [Preprint]. Preprints.org.

Naziyah, Hidayat, R., & Maulidya. (2022). Penyuluhan manajemen luka terkini dalam situasi pandemi COVID-19 melalui kegiatan pesantren luka dengan menggunakan media Zoom Meeting bagi mahasiswa Prodi Keperawatan & Profesi Ners Fakultas Ilmu Kesehatan Universitas Nasional Jakarta. Jurnal Kreativitas Pengabdian Kepada Masyarakat, 5(7), 1–10.

Proenza, Y. G., López, M. G., & Valdés, J. A. (2013). Chemical composition and antibacterial activity of the essential oil from Eucalyptus pellita F. Muell. Journal of Medicinal Plants Research, 7(27), 1979–1983.

Putri, P. W. D., Maheswari, A. P., & Pratiwi, L. (2023). Manfaat ekstrak kunyit (Curcuma domestica Val.) dalam menghambat pertumbuhan Staphylococcus aureus. E-Jurnal Medika Udayana, 12(3), 99–105.

Rajendran, S. (2018). Advanced Textiles for Wound Care. Cambridge: Woodhead Publishing.

Salehi, B., Sharifi-Rad, J., Seca, A. M. L., Pinto, D. C. G. A., Michalak, I., & Martins, N. (2019). Insights into Eucalyptus genus: Chemical constituents, biological activities and health-promoting effects. Trends in Food Science & Technology, 91, 609–624.

Saptowo, A., & Supriningrum, R. (2021). Uji aktivitas antibakteri ekstrak kulit batang sekilang (Embelia borneensis Scheff) terhadap bakteri Propionibacterium acnes dan Staphylococcus epidermidis. Jurnal Farmasi Borneo, 1(2), 93–97.

Shrivastav, A., Kim, H., & Kim, S. (2018). In vivo models for assessment of wound healing potential: A systematic review. Wound Medicine, 20, 43–53.

Toumi, M. N., Djebli, N., & Boulekbache-Makhlouf, L. (2022). Intensification of the extraction yield of Eucalyptus globulus phenolic compounds with pulsed electric field. Applied Sciences, 12(15), 7893.

Wintachai, P., Paosen, S., & Takahashi, C. (2019). Silver nanoparticles synthesized with Eucalyptus citriodora ethanol leaf extract stimulate antibacterial activity against clinically multidrug-resistant

Acinetobacter baumannii isolated from pneumonia patients. Microbial Pathogenesis, 126, 245–257.

Yuan, G., Peng, Y., Yuan, J., & Wang, Q. (2021). Antibacterial activity and mechanism of plant flavonoids to Gram-positive bacteria predicted from their lipophilicities. Scientific Reports, 11, Article 10471.




DOI: https://doi.org/10.33024/jikk.v13i4.21893

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