Uji Efektifitas Antiinflamasi Sediaan Ko-Amorf Ketoprofen-L-Arginin Dengan Metode Paw Edema Pada Tikus Wistar Jantan

Wahyu Alfath Firdaus, Salman Umar, Erizal Zaini

Sari


Inflamasi merupakan suatu bentuk respon terhadap kerusakan jaringan pada tubuh. Ketoprofen merupakan golongan senyawa antiinflamasi non-steroid (NSAID) yang memiliki kelarutan yang rendah dalam air. Formulasi ko-amorf ketoprofen dan l-arginin dengan perbandingan molar 1:1 mampu memperbaiki kelarutan dan disolusi ketoprofen. Ko-amorf ketoprofen-l-arginin diuji aktifitasnya dengan metode Paw Edema. Sediaan diujikan kepada tikus jantan galur Wistar dengan bobot sekitar 200 g. Penelitian dilakukan terhadap 15 tikus yang dibagi ke dalam tiga kelompok perlakuan; suspensi CMC-Na 0,5%; suspensi ketoprofen murni dengan dosis 3,6 mg/200 gBB; suspensi ko-amorf ketoprofen-l-arginin 5,3 mg Kg/200 gBB (setara 3,6 mg/200 gBB ketoprofen murni). Pemberian obat diberikan secara per oral, 30 menit kemudian diikuti induksi karagenan 1% secara intraplantar untuk memicu edema. Pengukuran volume edema dilakukan setiap 60 menit selama 360 menit menggunakan alat plethysmometer. Data volume udem diubah menjadi persentase rata-rata volume udem dianalisis secara statistik menggunakan SPSS. Hasil penelitian menunjukkan suspensi ko-amorf ketoprofen – L-arginin 1:1 mampu menurunkan volume edema pada kaki tikus Wistar jantan dibandingkan suspensi ketoprofen murni dan CMC-Na 0,5%.


Kata Kunci


Ko-Amorf; Ketoprofen; L-Arginin; Laju Disolusi; Uji Antiinflamasi

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Referensi


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DOI: https://doi.org/10.33024/jikk.v12i12.23675

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